Iningainema tapete sp. nov. (Scytonemataceae, Cyanobacteria) from greenhouses in central Florida (USA) produces two types of nodularin with biosynthetic potential for microcystin-LR and anabaenopeptin production

Iningainema is a recently described genus of heterocytous, false-branching cyanobacteria originally from Australia. In this work, we present tapete sp. nov., isolated subaerial and terrestrial environments in central Florida (USA). comparison to the sister species, our novel cyanobacterium produces nodularin-R (NOD-R) methylated isoform [MeAdda3] NOD previously not reported within genus; addition possessing biosynthetic gene clusters for microcystin anabaenopeptins production. Nodularin accumulation by exceeded 500 µg g−1 dry weight cultures grown nitrogen-depleted media. Such elevated toxin concentrations are alarming as was food production greenhouse poses potential risk products workforce exposure. Using morphology, 16S rRNA phylogeny, 16S-23S internal transcribed spacer (ITS) secondary structure, coupled with detection presence, provide evidence establishment toxic species cyanobacteria, tapete.